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URL: http://www.addgene.org/165553
Proper Citation: RRID:Addgene_165553
Insert Name: Bst DNA Polymerase, Large Fragment
Organism: Other
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pBuild; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: Fragment retains 5'-3' polymerase activity from full length Bst DNA Polymerase, while lacking 5'-3' exonuclease activity. Suitable for applications requiring thermophilic strand displacement. Additionally used for Loop-Mediated Isothermal Amplification (LAMP), DNA sequencing through high GC regions, rapid sequencing from nanogram amounts of DNA template. Can be used in the standard Sanger one step or two step protocol which separates the labelling reaction from the elongation termination reaction. The enzyme can be used in double stranded sequencing. The plasmids in the Open Enzyme collection contain genetic parts and are not functional by themselves. Researchers can clone these enzymes into an expression vector of their choice and then transform into E. coli bacteria for expression and purification. Additional information can be found at https://www.addgene.org/depositor-collections/open-enzyme/.
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Source: Addgene
