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URL: http://www.addgene.org/166756
Proper Citation: RRID:Addgene_166756
Insert Name: Venus
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35442739
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid can be used to express monomeric Venus fluorophore in bacteria. We used E.coli BL21-AI (Arabinose induction) for protein production, induced 5h at 30oC. Purify protein via Nickel column chromatography. When compared with EYFP, Venus fluorophore contains the following mutations: F46L, F64L, M153T, V163A and S175G. This Venus protein contains an F224R mutation to make it monomeric.
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Source: Addgene
