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URL: http://www.addgene.org/16881
Proper Citation: RRID:Addgene_16881
Insert Name: isthmin
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:15797017
Vector Backbone Description: Backbone Size:4100; Vector Backbone:pSPORT1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Zebrafish Isthmin cDNA, clone 27j15 from the microarray screen 1st round (Matthew Clark normalized late somitogenesis / 24 hpf / liver library) in pSPORT1. A clone that is slightly longer at the 5' end was identified in the 2nd array round, so this is not exactly full-length. For antisense probe synthesis. Expressed on dorsal side early, in a dorsal high to ventral low gradient in margin, during gastrulation around axial mesoderm, later strongly at MHB. For antisense: Asp718, SP6. Citation: G. Weidinger and R. T. Moon (2005). Development.
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Source: Addgene
