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URL: http://www.addgene.org/17059
Proper Citation: RRID:Addgene_17059
Insert Name: sonic hedgehog
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:7583153
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pT7TS; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Steve Ekker clone E, in vector pT7TS. Linearize with BamH1 and transcribe with T7 for sense RNA for injections. Citation: Ekker et al. (1995), Current Biology 5:944-955. Made by insertion of shh open-reading frame into the SpeI site of the pT7TS vector. The ORF was made by PCR using the following primers: shhA (5′-GGACTAG-TCACCATGCGGCTTTTGACGAGA-3′) and shhB (5′-AGCATACTAGTAGTCTCAGCTTGAGTTTACTGA-3′).
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Source: Addgene
