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Plasmid Name
M16 pCS-twhh-bGFP
RRID:Addgene_17153 RRID Copied  
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RRID:Addgene_17153
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Plasmid Information

URL: http://www.addgene.org/17153

Proper Citation: RRID:Addgene_17153

Insert Name: twhh promoter

Organism: Danio rerio

Bacterial Resistance: Ampicillin

Defining Citation: PMID:9314535

Vector Backbone Description: Backbone Size:0; Vector Backbone:pCS; Vector Types:; Bacterial Resistance:Ampicillin

Comments: Insert: twhh promoter (5.2 Kb), bGFP (1 Kb) Plasmid History: The reporter gene, "bright" green fluorescent protein (bGFP) with a serine 65 to threonine mutation (Heim et al., 1995), was cloned into vector pCS-twhh-beta -gal-vec BamHI/XhoI sites by blunt end ligation. The resulting plasmid was named pCS-twhh-bGFP. Notes for Use: Gives notochord-specific expression of GFP. Citation: Du et al., 1997, J. Cell Biology.

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Data and Source Information

Source: Addgene