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URL: http://www.addgene.org/17155
Proper Citation: RRID:Addgene_17155
Insert Name: twhh promoter
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9314535
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCS-NBeta gal; Vector Types:; Bacterial Resistance:Ampicillin
Comments: Parent vector: pCS-twhh-nBeta gal. Insert: twhh promoter (5.2 Kb), nBeta gal (3 Kb) Plasmid History: Source: zebrafish. Notes for Use: To make the twhh promoter into a convenient expression vector for expressing heterologous cDNAs, the BamHI site upstream of the twhh promoter in the plasmid pCS-twhh-beta -gal was deleted. The resulting plasmid, which retains the BamHI site between the promoter and the beta -gal, was isolated and designated pCS-twhh-beta -gal-vec. Genes of interest can be cloned into the BamHI and XhoI sites of this vector by replacing the beta -gal sequence. Citation: Du et al., 1997, J. Cell Biology.
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Source: Addgene
