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URL: http://www.addgene.org/17209
Proper Citation: RRID:Addgene_17209
Insert Name: Sp5l promoter
Organism: Danio rerio
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: Description: a fragment of putative zebrafish Sp5l promoter - 1203bp upstream of the 1st Exon. (as defined by the 27i2 clone found in the microarray screen) - plus 51bp of 5'UTR from Exon1 clone. upstream of firefly luciferase in the pGL3 Basic promoter. 6 of the putative Tcf/Lef sites mutated. (all introduce additional restriction sites); cloned by PCR-based in vitro synthesis from 28 overlapping. oligos. cloned NdeI / HindIII to exchange wild-type fragment in pGL3 Sp5l P1203. Species of Sequence Origin: zebrafish.
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Source: Addgene
