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URL: http://www.addgene.org/173204
Proper Citation: RRID:Addgene_173204
Insert Name: ATP1A1 G7 sgRNA + SpCas9
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:36207338
Vector Backbone Description: Vector Backbone:pX330-U6-Chimeric_BB-CBh-hSpCas9; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: ATP1A1 G7 sgRNA target ATP1A1 intron 17 and this plasmid can be used to directly target a transgene to the ATP1A1 locus itselft, including the mTOR Signaling Indicator (TOSI) using ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor. This plasmid is derived from pX330-U6-Chimeric_BB-CBh-hSpCas9. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
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Source: Addgene
