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URL: http://www.addgene.org/173496
Proper Citation: RRID:Addgene_173496
Bacterial Resistance: Kanamycin
Defining Citation: PMID:35256751
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2200; Vector Backbone:pDONR221 P4r-P3r; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Comments: Golden Gate assemble your genes of interest using the type IIs BsaI (or Eco31I) restriction enzyme, which generates a TAGG-5' overhang on one side, and a 5'-GCTT overhang on the other side of the vector. In our protocols these overhangs are termed: ATCC, GCTT (only considering the upper DNA strand for simplicity).
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Source: Addgene
