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URL: http://www.addgene.org/17381
Proper Citation: RRID:Addgene_17381
Insert Name: ZAP
Organism: Rattus norvegicus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:12215647
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5100; Vector Backbone:pCDNA4 TO2 myc HisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The NZAP fragment was excised from pBabe-NZAP-Zeo with EcoRI-NotI and cloned into pCDNA4/TO2-myc-HisB to generate a myc-tagged NZAP. The C-terminal portion of ZAP was cloned from a Rat2 cell cDNA library by PCR using sense primer CZAP-SP (5’-GAGCTCTCTGGGCTTAACC-3’) and antisense primer CZAP-AP (5’- ATATAGGCGGCCGCCCTCTGGACCTCTTCTCTTC-3’). The sense primer lies upstream from an internal NheI site in NZAP; the antisense primer introduces a NotI site immediately downstream from the coding sequence to facilitate its cloning into the myc-tagged expression vector.
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Source: Addgene
