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URL: http://www.addgene.org/174373
Proper Citation: RRID:Addgene_174373
Insert Name: protein AG - hia5
Organism: Other
Bacterial Resistance: Kanamycin
Defining Citation: PMID:35396487
Vector Backbone Description: Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Protocol for purification and expression can be found at: dx.doi.org/10.17504/protocols.io.bv82n9ye Application for DiMeLo-seq is described in: doi.org/10.1101/2021.07.06.451383 Protocol was adapted and modified from: doi.org/10.1126/science.aaz1646 This plasmid has been observed to occur in our stock as a plasmid dimer. Dimerization often does not impact plasmid function, but may reduce transformation and transfection efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid in a recombinase-deficient strain.
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Source: Addgene
