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URL: http://www.addgene.org/178109
Proper Citation: RRID:Addgene_178109
Insert Name: ATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:36207338
Vector Backbone Description: Vector Backbone:pU6-pegRNA-GG-acceptor; Vector Types:Mammalian Expression, CRISPR, Prime editing; Bacterial Resistance:Ampicillin
Comments: Control pegRNA vector derived from ATP1A1_G6_T804N_Dual_pegRNA. This vector can be used as a positive control for prime editing (PE3 in combination with ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA) to co-target ATP1A1 (T804N) and MTOR to perform coselection for MTOR rapamycin resistance mutation F2108L. Please visit https://doi.org/10.1101/2021.11.02.464583 for bioRxiv preprint.
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Source: Addgene