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URL: http://www.addgene.org/182506
Proper Citation: RRID:Addgene_182506
Bacterial Resistance: Kanamycin
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET24; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Primers for LIC cloning: Upstream: add TATTTTCAATCCTACGTA to the 5’ end of gene of interest. Downstream: add CCCTCAATATTATACGGG to the 3’ end of GOI. Detailed cloning method available in the paper Bruni R, Kloss B. High-throughput cloning and expression of integral membrane proteins in Escherichia coli. Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34.
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Source: Addgene
