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Plasmid Name
pFastBac1-pGC-A
RRID:Addgene_186626 RRID Copied  
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RRID:Addgene_186626
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Plasmid Information

URL: http://www.addgene.org/186626

Proper Citation: RRID:Addgene_186626

Insert Name: NPR1

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:35821229

Vector Backbone Description: Backbone Marker:Invitrogen; purchased via GenScript; Backbone Size:4682; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin

Comments: N-terminal protein sequence is MKTIIALSYIFCLVFA (hemagglutinin [HA] signal peptide) + HHHHHHHHHH + GAP (from AscI site) + ENLYFQ*G (TEV protease cleavage site; cleavage at * leaves an extra G residue at the N-terminus of pGC-A). Backbone vector is pFastBac1. Construct design by Shangji Zhang. Generate the bacmid in Escherichia coli strain DH10Bac cells according to the instructions from Invitrogen.

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Data and Source Information

Source: Addgene