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URL: http://www.addgene.org/18785
Proper Citation: RRID:Addgene_18785
Insert Name: TRAP
Organism: Bacillus subtilis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:17023487
Vector Backbone Description: Backbone Size:4200; Vector Backbone:n/a; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Mammalian expression vector for the expression of HA and His6 epitope-tagged TRAP. There is a single amino acid (glycine) insertion between translational initiator codon and second amino acid of TRAP (asparagine) to maintain Kozak consensus sequence. Also, there is a single amino acid difference at amino acid #60 which has an asparagine (N) as in Bacillus amyloliquefaciens rather than glutamic acid (E) as in Bacillus subtilis. Codons are a mixture of human, Bacillus amyloliquefaciens, and Bacillus subtilis.
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Source: Addgene
