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Plasmid Name
pTBS 5Y YIC
RRID:Addgene_18786 RRID Copied  
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RRID:Addgene_18786
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Plasmid Information

URL: http://www.addgene.org/18786

Proper Citation: RRID:Addgene_18786

Insert Name: TRAP binding site

Bacterial Resistance: Kanamycin

Defining Citation: PMID:17023487

Vector Backbone Description: Backbone Marker:Available at Addgene (#18673); Backbone Size:6270; Vector Backbone:pYIC; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin

Comments: An oligonucleotide with a 55-nt TRAP-binding sequence, TBS, was inserted into the 5'UTR of pYIC DNA to generate a bicistronic reporter gene with TBS: The TBS was inserted in the SacI site for 5-UTR (pTBS/5Y-YIC; 45 nt downstream of transcription start site and 9 nt upstream of YFP translational start site).

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Data and Source Information

Source: Addgene