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URL: http://www.addgene.org/190175
Proper Citation: RRID:Addgene_190175
Insert Name: sgRNA cloning site
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:36287051
Vector Backbone Description: Backbone Marker:Addgene #60847; Vector Backbone:pCAS; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid is a modified version of pCAS (Addgene #60847) that has been improved to facilitate rapid cloning of new gRNA sequencing for streamlined CRISPR-Cas9 genome editing in yeast. pCAS was originally published by: Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife.03703.
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Source: Addgene