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URL: http://www.addgene.org/191794
Proper Citation: RRID:Addgene_191794
Insert Name: TRP1 pCUP1 with 2 phlO sites
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:pFA6a; Vector Types:Yeast genomic targeting; Bacterial Resistance:Ampicillin
Comments: pFA6a-TRP1-pCUP1-2xphlO includes phlO operator sites inserted in the CUP1 promotor to allow binding and transcriptional repression by the phlF repressor protein. This repression is relieved by addition of 2,4-Diacetylphloroglucinol (DAPG) to the medium. The system is modeled after one described in the following manuscript by Ikushima and Boeke of Johns Hopkins University. New Orthogonal Transcriptional Switches Derived from Tet Repressor Homologues for Saccharomyces cerevisiae Regulated by 2,4-Diacetylphloroglucinol and Other Ligands Shigehito Ikushima and Jef D. Boeke ACS Synthetic Biology 2017 6 (3), 497-506 DOI: 10.1021/acssynbio.6b00205
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Source: Addgene
