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URL: http://www.addgene.org/195609
Proper Citation: RRID:Addgene_195609
Insert Name: PI-SceI
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:7890714
Vector Backbone Description: Vector Backbone:pT7-7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Contains a D326A mutation in the second LAGLIDADG motif that inactivates the protein.
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No rating or validation information has been found for pT7PI-Sce ESARC D326A.
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Source: Addgene
