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URL: http://www.addgene.org/195613
Proper Citation: RRID:Addgene_195613
Insert Name: I-SceI
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:18644379
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The protein contains a carboxyl-terminal histidine tag for purification. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur).
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No rating or validation information has been found for pET15b I-SceI (Ctermhis -MH).
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Source: Addgene
