Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://www.addgene.org/199223
Proper Citation: RRID:Addgene_199223
Insert Name: Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:36928106
Vector Backbone Description: Backbone Size:2700; Vector Backbone:pBR322; Vector Types:Mammalian Expression, Gene targeting donor plasmid; Bacterial Resistance:Ampicillin
Comments: This target site-containing donor plasmid can be used to insert a transgene at the human CCR5 safe harbor locus when delivered together with gRNA expression plasmid AY22_pgRNA.R5.1 (Addgene plasmid #100294) and a SpCas9 nuclease (HMEJ-based gene targeting) or a SpCas9.D10A nickase (ITPN-based gene targeting). Note: to reduce the chances for off-targeting donor DNA insertions when applying HMEJ-based genome editing it is preferable using AW01_pU.CAG.eSpCas9(1.1).rBGpA (Addgene plasmid #199255) or a similar construct, i.e., expressing a high specificity nuclease. Combining this donor with a S. pyogenes Cas9 nickase (e.g., Addgene plasmids #199256, #199253, #199254 or #199252) leads to seamless ITPN-mediated gene targeting.
Expand AllWe found {{ ctrl2.mentions.all_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for BB44_pmc.DonorR5.TS.
No alerts have been found for BB44_pmc.DonorR5.TS.
Source: Addgene
