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URL: http://www.addgene.org/200050
Proper Citation: RRID:Addgene_200050
Insert Name: BCD 8.5K
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:37253269
Vector Backbone Description: Vector Backbone:pUC; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Comments: This plasmid encodes a part for use in a heirarchical golden gate plasmid assembly system. The part insert is a bicistronic translational control element for regulating translation of protein-coding genes in E. coli. It is flanked by sites for the type IIS restriction enzyme BsaI and yields 5' and 3' overhangs of GGAG and TATG respectively. The backbone contains a pUC origin, a TEM-1 ampicillin resistance marker, and a ccdB marker such that the part plasmid does not carry through into higher order plasmid assemblies. The plasmid must be propagated in an E. coli strain which is resistant to ccdB. Please visit https://www.biorxiv.org/content/10.1101/2023.02.09.527918v1 for bioRxiv preprint.
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Source: Addgene
