Searching the RRID Resource Information Network
Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.
URL: http://www.addgene.org/200191
Proper Citation: RRID:Addgene_200191
Insert Name: blasticidine - P2A - TETon-3G transactivator
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:36307419
Vector Backbone Description: Backbone Marker:Vlatka Zoldos; Backbone Size:8020; Vector Backbone:#71666 pdCas9-DNMT3A-EGFP; Vector Types:Mammalian Expression, Bacterial Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: A lentiviral UbC-blasticidine-P2A-TETon-3G transactivator vector was synthesized using the #71666 pdCas9-DNMT3A-EGFP vector backbone digested with NheI-EcoRI and ligated to the following DNA fragments: UbC promoter and blasticidine cassette amplified and fused by PCR using 40–50 bp internal overlapping primers and one external reverse primer containing a P2A linker, and a TETon-3G fragment amplified from the Addgene vector #96963 pCAG-TetON-3G using a forward primer containing a P2A sequence so it could be fused to the UbC-Blasticidin-P2A sequence during a last round PCR amplification with NheI and EcoRI external primers.
Expand AllWe found {{ ctrl2.mentions.all_count }} mentions in open access literature.
We have not found any literature mentions for this resource.
We are searching literature mentions for this resource.
Most recent articles:
{{ mention._source.dc.creators[0].familyName }} {{ mention._source.dc.creators[0].initials }}, et al. ({{ mention._source.dc.publicationYear }}) {{ mention._source.dc.title }} {{ mention._source.dc.publishers[0].name }}, {{ mention._source.dc.publishers[0].volume }}({{ mention._source.dc.publishers[0].issue }}), {{ mention._source.dc.publishers[0].pagination }}. (PMID:{{ mention._id.replace('PMID:', '') }})
Check
for all resource mentions.
A list of researchers who have used the resource and an author search tool
A list of researchers who have used the resource and an author search tool. This is available for resources that have literature mentions.
No rating or validation information has been found for UbC-blasticidine-P2A-TETon-3G transactivator.
No alerts have been found for UbC-blasticidine-P2A-TETon-3G transactivator.
Source: Addgene
