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URL: http://www.addgene.org/200227
Proper Citation: RRID:Addgene_200227
Insert Name: tRNA-gRNA scaffold
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Kanamycin
Defining Citation: PMID:37326023
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3500; Vector Backbone:pCR-Blunt II-TOPO; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: A common PCR template used fo CRISPR Engineering of EPisomes in Yeast, or CREEPY, a method for rapid engineering of large synthetic episomal DNA constructs. This is designed and assembled based on the template consisting of gRNA scaffold with a tRNA sequence, as published in Zhang, Yueping, et al. "A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae." Nature communications 10.1 (2019): 1053.
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Source: Addgene
