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Plasmid Name
RRID:Addgene_213026 RRID Copied  
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RRID:Addgene_213026
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Plasmid Information

URL: http://www.addgene.org/213026

Proper Citation: RRID:Addgene_213026

Insert Name: Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Defining Citation: PMID:38096902

Vector Backbone Description: Backbone Marker:Invitrogen (modified); Backbone Size:9879; Vector Backbone:pCEP4 Puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin

Comments: This vector is similar to pLD401 (Taylor et al. Cell 2013) but the C-terminal tag on ORF2p is simpler. The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2).

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Ratings and Alerts

No rating or validation information has been found for pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro.

No alerts have been found for pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro.

Data and Source Information

Source: Addgene