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URL: http://www.addgene.org/213031
Proper Citation: RRID:Addgene_213031
Insert Name: Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:38096902
Vector Backbone Description: Backbone Marker:Genscript / Invitrogen; Backbone Size:8859; Vector Backbone:pCEP Puro; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: The construct is based on the synthetic L1 ORFeus-Hs sequence generated in the lab of Jef Boeke (see W. An et al., Mobile DNA 2, 2011 https://doi.org/10.1186/1759-8753-2-2). For integration with Sleeping Beauty such as SB100x (Izsvak lab, Addgene #34879). This vector is similar to pYX056 from An lab, see also: Xie, Y. et al. Cell division promotes efficient retrotransposition in a stable L1 reporter cell line. Mob DNA 4, 10 (2013). https://doi.org/10.1186/1759-8753-4-10 and Xie, Y., Rosser, J. M., Thompson, T. L., Boeke, J. D. & An, W. Characterization of L1 retrotransposition with high-throughput dual-luciferase assays. Nucleic Acids Res 39, e16 (2011). https://doi.org/10.1093/nar/gkq1076
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Source: Addgene
