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URL: http://www.addgene.org/218513
Proper Citation: RRID:Addgene_218513
Insert Name: ffluc
Organism: Photinus pyralis
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:38911550
Vector Backbone Description: Vector Backbone:pSpy1C; Vector Types:Bacterial Expression, Luciferase; Bacterial Resistance:Chloramphenicol
Comments: This high-copy replicative shuttle plasmid features an erythromycin-inducible riboswitch driving expression of the Firefly luciferase in the presence of erythromycin. Luminescence can be detected by adding luciferin to the culture. See associated publication for more details. Resource Information: The erythromycin-inducible riboswitch was taken from pLZ12Km2-TA:PgyrA(Sag)-ermBL-ermB':A2I:ffluc-tt3, which was used in the following publication: Dynamics of diversified A-to-I editing in Streptococcus pyogenes is governed by changes in mRNA stability Thomas F. Wulff, Karin Hahnke, Anne-Laure Lécrivain, Katja Schmidt, Rina Ahmed-Begrich, Knut Finstermeier, Emmanuelle Charpentier bioRxiv 2023.09.19.555891; doi: https://doi.org/10.1101/2023.09.19.555891 The ffluc reporter gene was taken from the original pLZ12Km2-P23R:TA:ffluc plasmid (Plasmid #88900). Please download the detailed plasmid map using the file linked below
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No rating or validation information has been found for pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc).
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Source: Addgene
