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URL: http://www.addgene.org/221114
Proper Citation: RRID:Addgene_221114
Insert Name: MFA-IgKappa-FAPoptim-STE3
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:38809589
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: The pRS415-TEF1pr-MFA-NFAPoptim plasmid was used to insert the Ste3 coding sequence as an in-frame N-terminal fusion to FAP. The Ste3 coding sequence (lacking the stop codon) was inserted into the SmaI and XhoI restriction enzyme sites. The resulting plasmid contains the MFA1 ER targeting and secretion sequence, an N-terminal FAPoptim tag on Ste3.
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Source: Addgene
