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URL: http://www.addgene.org/223321
Proper Citation: RRID:Addgene_223321
Insert Name: SpCas9 codon optimized for human
Organism: Homo sapiens
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:39721357
Vector Backbone Description: Vector Backbone:pX330_42230; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Expression vector from Cong et al. 10.1126/science.1231143 that carries the chloramphenicol resistance protein ABO28845.1 and the negative selection marker ccdB ABO28846.1 for efficient cloning of sgRNA targeting sequences.
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No rating or validation information has been found for pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9.
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Source: Addgene
