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URL: http://www.addgene.org/224697
Proper Citation: RRID:Addgene_224697
Insert Name: SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
Organism: SARS-CoV-2 virus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:39673710
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Silent mutation in the original sequence to delete a second NdeI site as NdeI was needed for cloning; codon coding for Q306 was mutated to codon coding for A306 to delete natural cleavage site of the 3CL-protease; two codons for glycine as a linker before the c-terminal 6x histag.
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Source: Addgene
