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URL: http://www.addgene.org/232751
Proper Citation: RRID:Addgene_232751
Insert Name: p53DD
Organism: Mus musculus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:42062565
Vector Backbone Description: Backbone Size:1600; Vector Backbone:pENTR4; Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Comments: The 5' and 3' beta globin UTR sequences flanking the ORF were based on previous literature for effective modRNA expression in hiPSCs: 10.1016/j.crmeth.2022.100290 To generate linear IVT template, digest the plasmid with MluI. The vector encodes a segmented polyA track, obviating the need for polyadenylation after IVT (doi: 10.1261/rna.069286.118). Due to the inability of nanopore sequencing to accurately read long polyN stretches and the possibility of polyA truncation, the actual length of the polyA tail may differ when propagating the vector. In our hands, we have not seen measurable differences in expression across different plasmid preparations.
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Source: Addgene
