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URL: http://www.addgene.org/240639
Proper Citation: RRID:Addgene_240639
Insert Name: AIPGENE865 promoter
Organism: Exaiptasia diaphana
Bacterial Resistance: Ampicillin and Kanamycin
Vector Backbone Description: Backbone Marker:Lab of Ulrich Technau; Backbone Size:4115; Vector Backbone:pNvT-MHC::mCh; Vector Types:Expression of NLS-Kaede in Exaiptasia diaphana; Bacterial Resistance:Ampicillin and Kanamycin
Comments: The whole intergenic region between AIPGENE865 (KXJ12416.1; Elongation Factor 1alpha) and AIPGENE899 was cloned as promoter fragment. The original gene annotation for AIPGENE865 is incorrect, the automatic NCBI annotation is correct in regards of exons, introns and translation start site. We found that Aiptasia strain CC7 was actually heterozygous for that locus: one allele was mostly in agreement with the genome sources but the other one had a couple of SNPs and a large, 749-bp insert in the intergenic region. The longer version with insert was used as promoter for this plasmid.
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Source: Addgene
