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URL: http://www.addgene.org/24347
Proper Citation: RRID:Addgene_24347
Insert Name: GAL80
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20434990
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5493; Vector Backbone:pcDNA3.1-Myc-His-A; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: GAL80 cDNA was PCR amplified using primers PR511 (CCCCGGATCCCAACatggactacaacaagagatcttcgg) and PR512 (CGGTTAACGCGGCCGCttataaactataatgcgagatattgctaacg), and a lab vector, pCA-GAL80 as the template. The PCR product was cloned using BamHI and NotI into pCDNA3.1-myc- His-A. A stop codon precedes the Myc and His tags.
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Source: Addgene