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URL: http://www.addgene.org/24590
Proper Citation: RRID:Addgene_24590
Insert Name: Gateway(TM) cassette
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: pLD-hygro-EnVM was constructed by subcloning the hygromycin resistance gene from the pLJM6 vector (J Moffat, unpublished) into the pLD-puro-EnFH vector using SpeI/NsiI and subsequently subcloning a gene synthesized V5-Myc (VM) tag (Bio Basic Inc.) from the pUC57 host vector. pLD-puro-EnFH (pLD-puromycin resistance- EF1alpha, N-terminus triple Flag-6-His) was generated from pLKO.1 (see paper for details).
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Source: Addgene
