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URL: http://www.addgene.org/246980
Proper Citation: RRID:Addgene_246980
Insert Name: FLP Recombinase
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Kanamycin
Vector Backbone Description: Backbone Marker:NA; Backbone Size:5209; Vector Backbone:pET28a(+)v2; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Comments: We use a tag-free SUMO protease (ulp1) for cleaving the TwinStrep tag from the final product. Tag-free ulp1 can be synthesized using Addgene Plasmid #190063 and a His-tagged TEV protease or HRV-3C protease (PreScission protease). The total insert size displayed on this page (1872bp) includes all tags and linkers.
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Source: Addgene
