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Plasmid Name
EKv376(gRNAscaffold-tRNA for cloning)
RRID:Addgene_248333 RRID Copied  
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RRID:Addgene_248333
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Plasmid Information

URL: http://www.addgene.org/248333

Proper Citation: RRID:Addgene_248333

Insert Name: gRNAscaffold-tRNA

Organism: P. patens

Bacterial Resistance: Ampicillin

Vector Backbone Description: Vector Backbone:pUC18; Vector Types:CRISPR; Bacterial Resistance:Ampicillin

Comments: Golden Gate fragments are amplified by PCR, with primers designed to produce half of each gRNA followed by BsaI recognition sites. Complete gRNA sequences to be reconstituted upon BsaI digestion and ligation during the Golgen Gate reaction.

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Data and Source Information

Source: Addgene