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URL: http://www.addgene.org/254139
Proper Citation: RRID:Addgene_254139
Insert Name: SUMO family protein SMT3
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Kanamycin
Defining Citation: PMID:41927759
Vector Backbone Description: Backbone Size:5228; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This plasmid contains a SUMO protein construct to serve as a control protein for binding experiments using the AH-SUMO platform, to verify non-interaction between the SUMO domain and liposomes of choice. It contains the SUMO1-98 region of the SMT3_yeast gene, followed by a Cysteine, a TEV site and a hexa-histidine site. On the N-terminal of the SUMO domain, it contains a region encoding for MGSGS, which is there to mimic the structure of a AH-SUMO platform that contains an N-terminal peptide of interest , serving as a better control. Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint.
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No rating or validation information has been found for pET29b(+)_SUMO(1-98)_sTEV_His6.
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Source: Addgene
