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URL: http://www.addgene.org/254142
Proper Citation: RRID:Addgene_254142
Insert Name: NcoI_SMT3-SUMO(21-98)_C_sTEV_His6
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Kanamycin
Defining Citation: PMID:41927759
Vector Backbone Description: Backbone Size:5237; Vector Backbone:pET29b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: This vector is a modified pET29b(+) vector that includes one added cloning site (NcoI) followed by the encoding region for a fragment of the SMT3_yeast gene (21-98) followed by a TEV site (ENLYFQS) and a hexa-histidine site (HHHHHH*). Any peptide of interest can be cloned in the vector using the two cloning sites (NdeI and NcoI) via HiFi assembly. When adding a peptide of interest this way, the remaining N-terminal portion of the SMT3 gene should be added as well, separated by the peptide of interest with a GSGS encoding region (GSGSMSDSEVNQEAKPEVKPEVKP). This can result in a vector which encodes for the expression of a recombinant gene of the following type : PEPTIDE-GSGS-SUMO(1-98)-C-sTEV_His6*. Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint.
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Source: Addgene
