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URL: http://www.addgene.org/25423
Proper Citation: RRID:Addgene_25423
Insert Name: Myo D 5' flanking sequence
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:17693064
Vector Backbone Description: Backbone Size:4200; Vector Backbone:pEGFP-1; Vector Types:Mammalian Expression, Reporter construct; Bacterial Resistance:Kanamycin
Comments: The MyoDGFP (-24GFP) transgene was constructed by excising 24 kb of human MyoD 5' flanking sequence from -24lacZ (Chen et al., 2001; Addgene plasmid #25422) by digestion with SalI and AscI (AscI linkers were added to the unique XhoI site at the 3' end of human genomic sequences) and inserting into SalI and AscI (produced by linker addition to the unique SmaI site) sites in the polylinker of pEGFP-1 (Clontech). Prior to subcloning, the NotI site just 3' of EGFP was destroyed by blunt-ending with Klenow, and a new NotI site was introduced by linker addition to the AflII site, which lies 3' of SV40 poly sequences.
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Source: Addgene
