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URL: http://www.addgene.org/26061
Proper Citation: RRID:Addgene_26061
Insert Name: Cyclin B1
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10395539
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pCMX; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: The stop codon of cyclin B1 in the pCMX vector was mutated to a Mfe1 site to link cyclin B1 to MmGFP. Cyclin B1 was fused to the second amino acid residue of MmGFP by a 5 amino acid linker (AGAQF). The construct is cloned as a HindIII-BamHI fragment into pCMX. The Cyclin insert contains a V5A polymorphism which has no functional consequence. More information on MmGFP can be found in the following reference: Zernicka-Goetz, M., Pines, J., M., Ryan, K., Siemering, K.R., Haseloff, J., and Gurdon, J.B. (1996) ‘An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
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No rating or validation information has been found for pCMX/cyclinB1-GFP (27).
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Source: Addgene
