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URL: http://www.addgene.org/26393
Proper Citation: RRID:Addgene_26393
Organism: Caenorhabditis elegans
Bacterial Resistance: Ampicillin
Defining Citation: PMID:20729841
Vector Backbone Description: Backbone Size:0; Vector Backbone:pBS; Vector Types:Bacterial Expression, Worm Expression; Bacterial Resistance:Ampicillin
Comments: PLEASE NOTE: this plasmid is approximately 1.6kb shorter than expected in the region between the T7 promoter and pBR322 origin region based on the author sequence. Both promoters are present in their entirety based on the Addgene Quality Control sequencing. According to the depositing laboratory the plasmid behaves as expected and gives the expected pattern of fluorescence and Neomycin resistant phenotype. There are a few other mismatches with author sequence but they also do not affect function. For questions regarding this plasmid please contact the depositing scientist Denis Dupuy at [email protected] AgeI is NOT a unique restriction site. A second site is present at around nucleotide 7210.
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Source: Addgene