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URL: http://www.addgene.org/26798
Proper Citation: RRID:Addgene_26798
Insert Name: multiple cloning sequence
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21375737
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Comments: Empty entry vector carrying Gateway L1L3 sites, with MCS. This plasmid can act as an empty carrier when combined with a R3L2 plasmid to generate expression plasmids carrying just the R3L2 component (i.e., TetO-transgene expressing plasmid) Plasmid was constructed as follows: The pEntr2B entry vector (Invitrogen) was digested with PstI and XhoI to remove the attL2 site. Oligonucleotides containing SalI-XhoI-Bsu36I-NdeI-PacI-EcoRI-NotI flanking the attL3 sequence on the 5' side and PstI on the 3' side were ligated in to create an intermediate plasmid carrying attL1 and attL3 sites. A LoxP sequence was introduced at the XhoI site.
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Source: Addgene
