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Plasmid Name
pEnt R3L2-MCS
RRID:Addgene_26799 RRID Copied  
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RRID:Addgene_26799
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Plasmid Information

URL: http://www.addgene.org/26799

Proper Citation: RRID:Addgene_26799

Insert Name: multiple cloning sequence

Bacterial Resistance: Kanamycin

Defining Citation: PMID:21375737

Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2100; Vector Backbone:pEntr2B; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin

Comments: Empty entry vector carrying Gateway R3L2 sites, with MCS. This plasmid can act as an empty carrier when combined with a L1L3 plasmid to generate expression plasmids carrying just the L1L3 component. This plasmid was constructed as follows: The pEntr2B entry vector was digested with AflII and XhoI to remove the attL1 and ccdb genes. Oligos containing the attR3 site flanked by AflII on the 5' end and a polylinker (NotI-PacI-Bsu36I-NdeI-XhoI) on the 3' end were ligated to generate pEntR3L2-MCS.

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Data and Source Information

Source: Addgene