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URL: http://www.addgene.org/27125
Proper Citation: RRID:Addgene_27125
Insert Name: Colicin Ia + Ia immunity protein
Organism: E. coli
Bacterial Resistance: Ampicillin
Defining Citation: PMID:19919671
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:PUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The original clone had some mutations that had been introduced during the PCR cloning of the colicin operon (into another vector). Here, the operon was moved into pUC19 and the mutations were corrected, so that the sequence is the same as the one in GenBank (M13819.1) This plasmid contains Colicin Ia + Ia immunity protein, each with their own promoters; colicin production can be induced with mitomycin C. The SOS promoter for colicin expression is at around residues 148-165 of the insert, in the forward direction. The immunity promoter -35 box is about 460 bp from the end of the insert; there are only about 20 bp between the terminator for the colicin gene, reading in one direction, and the terminator for the immunity protein, reading in the other.
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Source: Addgene
