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URL: http://www.addgene.org/27180
Proper Citation: RRID:Addgene_27180
Insert Name: lox/Frt/-PGK/EM7pm-G418/Kan-lox/Frt cassette flanked by Tn7L/R
Organism: synthetic
Bacterial Resistance: Tetracycline
Defining Citation: PMID:15784610
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:3000; Vector Backbone:pGPS21; Vector Types:in vitro transposion; Bacterial Resistance:Tetracycline
Comments: The plasmid confers Kan resistance (25ug/ml) and Tet resistance (12.5ug/ml). However, it requires pir factor for replication. Note that this plasmid is supplied on a stab with only Tet. When re-plating, end users should add Kan as well. Also note that the plasmid is only considered high copy in the pir-116 strain. In a WT pir strain, it is considered low copy.
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Source: Addgene
