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URL: http://www.addgene.org/27410
Proper Citation: RRID:Addgene_27410
Insert Name: SPL10 promoter
Organism: Arabidopsis thaliana
Bacterial Resistance: Kanamycin
Defining Citation: PMID:21123653
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCGTAG; Vector Types:; Bacterial Resistance:Kanamycin
Comments: SPL10p::NLS-GFP was generated by PCR amplification of 1.9-kb genomic DNA. The resulting amplicon was then cloned into pCR8/GW-TOPO (Invitrogen) and recombined with pCGTAG. The primers used to create this construct were 5'-TCATCATCTTTCACG-3' and 5'-TCAAGATGTGGGTTG-3'. Please note that Addgene's sequencing results identified single nucleotide mismatches at bp# 11592, 11985 and 12403 when compared to the full plasmid sequence provided by the depositing laboratory. These mismatches are not known to affect the plasmid function.
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Source: Addgene
