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URL: http://www.addgene.org/32166
Proper Citation: RRID:Addgene_32166
Insert Name: synthetic yeast Ubiquitin
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Vector Backbone Description: Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Comments: A ~1040 bp BamHI / HindIII fragment containing the CUP1 promoter, the synthetic yeast Ub gene and a CYC1 terminator sequence was ligated into the BamHI and HindIII sites of YEplac195. The resulting plasmid is approximately 6.281kb). K63A mutation was introduced by site-directed mutagenesis of LHP585 (Addgene 32168). 2 µm S. cerevisiae replication.
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No rating or validation information has been found for LHP809 - Ub-K63A-CYC1.
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Source: Addgene
