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URL: http://www.addgene.org/34625
Proper Citation: RRID:Addgene_34625
Insert Name: Myoglobin-127TAG
Organism: Sperm Whale: Physeter macrocephalus
Bacterial Resistance: Chloramphenicol
Defining Citation: PMID:21868676
Vector Backbone Description: Vector Backbone:pSepT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
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Source: Addgene
