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URL: http://www.addgene.org/35191
Proper Citation: RRID:Addgene_35191
Insert Name: loxP KAN loxP 24xPP7SL MDN1
Organism: Saccharomyces cerevisiae
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21512033
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR4; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid. Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert. Addgene's sequencing results identified a 3 nucleotide deletion at bp# 543-545 when compared to the full plasmid sequence provided by the depositing laboratory. This deletion is not known to affect plasmid function.
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No rating or validation information has been found for pDZ207 (loxP KAN loxP 24xPP7SL MDN1).
No alerts have been found for pDZ207 (loxP KAN loxP 24xPP7SL MDN1).
Source: Addgene