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Plasmid Name
pWPI-FLAG-hPRKCI
RRID:Addgene_35387 RRID Copied  
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RRID:Addgene_35387
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Plasmid Information

URL: http://www.addgene.org/35387

Proper Citation: RRID:Addgene_35387

Insert Name: Protein Kinase C iota

Organism: Homo sapiens

Bacterial Resistance: Ampicillin

Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:11076; Vector Backbone:pWPI; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin

Comments: Please note that the vector backbone, pWPI, is a bicistronic vector that allows for simultaneous expression of a transgene (PRKCI in this case) and an EGFP marker to facilitate tracking of transduced cells. The EGFP marker cDNA has been inserted downstream of EMCV IRES. The PRKCI insert in this plasmid is a known variant that lack the first nine amino acids of PRKCI when compared to GenBank entry NP_002731.4

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Data and Source Information

Source: Addgene